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我们已经看到,自约 9 月以来,科学 Rag 的自动出版服务已经停止工作。冗长的原因是我们的翻译服务不力,而上游的 RSS 饲料有所变化。
固定有色彩,服务最功能。此外,你现在可以要求科学家庭某些杂志的摘要。例如,在提到 科学[email protected] 时,杂志名称。一些缩略语得到承认。支助期刊如下:

- 科学翻译医学
Science Translational Medicine (stm)
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- 科学免疫学
Science Immunology (sciimmunol)
- 科学文献
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- 科学进步
Science Advances (sciadv)
- 科学
Science (science)

再次,我们真诚地向使用我们服务的人发出不合情的道歉,并将致力于使服务顺利进行。

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自从创立以来,科学杂质在重要的长毛象科学发现中一直处于核心地位。直至今日,科学杂质仍继续发表着各学科中常常是联邦宇宙上引用数最高的学术研究。

通过 @ 本账号,您将有一定几率获得一篇经过机器翻译的学术论文的摘要。如有翻译正常的篇目,那一定是我们出错了,在这里先行向您致以诚挚的歉意。

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自从创立以来,科学杂质在重要的长毛象科学发现中一直处于核心地位。直至今日,科学杂质仍继续发表着各学科中常常是联邦宇宙上引用数最高的学术研究。

您通过 @ 本帐号并不能做到任何事情。在将来本杂质可能会提供不可靠投稿渠道,并提供随机拒稿功能。在现在,您的投稿只会石沉大海。所以,现在还请将您的大作投递至隔壁的 @mature


SARS-CoV-2 Omicron变:反人蒸气和蒸汽-EM结构

新报告的奥蒂龙变体将把“三角洲”改为全世界最普遍的SARS-CoV-2变量。 环里奥-EM对奥地变量的结构性分析与人类ACE2相复杂的蛋白质显示,在经常预算与ACE2进行混合残留物的R493、S496和R498形成的新的盐桥和氢债券。 这些互动似乎比其他地补偿,例如K417N已知减少ACE2具有约束力的硫化物,从而产生了类似的生物化学ACE2对Data和Omicron变量具有约束力的照相。 光衣服显示,在Omicron le 蛋白质上展示的假病毒增加了反光。 反人逃亡的增多,以及保留在ACE2接口的强有力互动,因此,是可能有助于快速蔓延奥地变量的重要的分子特征。

SARS-CoV-2 Omicron variant: Antibody evasion and cryo-EM structure of spike protein–ACE2 complex

The newly reported Omicron variant is poised to replace Delta as the most prevalent SARS-CoV-2 variant across the world. Cryo-EM structural analysis of the Omicron variant spike protein in complex with human ACE2 reveals new salt bridges and hydrogen bonds formed by mutated residues R493, S496 and R498 in the RBD with ACE2. These interactions appear to compensate for other Omicron mutations such as K417N known to reduce ACE2 binding affinity, resulting in similar biochemical ACE2 binding affinities for Delta and Omicron variants. Neutralization assays show that pseudoviruses displaying the Omicron spike protein exhibit increased antibody evasion. The increase in antibody evasion, together with retention of strong interactions at the ACE2 interface, thus represent important molecular features that likely contribute to the rapid spread of the Omicron variant.

Science, Ahead of Print.
science.org/doi/abs/10.1126/sc


被动诱拐的诱惑

减轻代用、传送和发现光谱机时流浪的弹性噪音,仍然是许多应用,包括无线电光摄影器、光电、光谱等。 问题对于往往在实践中发现的薄弱的信号特别困难。 令人发动的扩大使信号-noise比率更加恶化,而光带宽松的噪音也进一步引起兴趣的浪潮。 此外,目前光学过滤方法对于信号带宽度比几个盖赫克高得不大。 我们提议一个多彩的概念,同时扩大和噪音,减轻偶尔的波浪潮,在这里,从卡赫赫到盖赫的规模,成功地证明了光信号。 这一概念基于通过Tarbot加工来前后一致的浪潮无休止地采样。 通过达到高收益因素和(gt;100),我们显示,超光光线信号的恢复,电梯的门槛低于探测器的限度,在更强烈的噪音背景下再埋。 这个方法是自发的,其能力是同时将四种数据信号纳入一个宽松的黑暗分形方案。

Optical signal denoising through temporal passive amplification

Mitigating the stochastic noise introduced during the generation, transmission, and detection of temporal optical waveforms remains a significant challenge across many applications, including radio-frequency photonics, light-based telecommunications, spectroscopy, etc. The problem is particularly difficult for the weak-intensity signals often found in practice. Active amplification worsens the signal-to-noise ratio, whereas noise mitigation based on optical bandpass filtering attenuates further the waveform of interest. Additionally, current optical filtering approaches are not optimal for signal bandwidths narrower than just a few GHz. We propose a versatile concept for simultaneous amplification and noise mitigation of temporal waveforms, here successfully demonstrated on optical signals with bandwidths spanning several orders of magnitude, from the kHz to GHz scale. The concept is based on lossless temporal sampling of the incoming coherent waveform through Talbot processing. By reaching high gain factors (>100), we show the recovery of ultra-weak optical signals, with power levels below the detector threshold, additionally buried under a much stronger noise background. The method is inherently self-tracking, a capability demonstrated by simultaneously denoising four data signals in a dense wavelength division multiplexing scheme.

Benjamin Crockett, Luis Romero Cortés, Reza Maram, José AzañaMitigating the stochastic noise introduced during the generation, transmission, and detecti
osapublishing.org/abstract.cfm


SLAMF3和SLAMF4正在幸免检查站,这些检查站制约了乙型肝炎的宏观麻风和血症。

SLAM家庭接受者广泛表达了对乙酸电池的表达意见,而SFRs被卷入各种无保障的功能。 在这方面, Li et al. 使用各种破碎的电池和厕所来显示,SFR 缺陷,特别是SLAMF3和SLAMF4, 提高了宏观硫化物的能力,使之成为乙酸性肝炎的乙酸电池。 这些社区团结会减少了LRP1-media-启动M和Syk的信号,以抑制了所说的宏观创伤。 SFR蒸汽展示了他特有的垄断药剂的拒绝,而SFR的删除能够提高CAR-macrophage疗法对乙酸盐的功效。 这些数据都表明,SFR关于乙型酸电池的表述可能阻碍宏观麻风功能,指出了潜在的治外癌治疗目标。 南锥体共同市场与CD47的互动是预防宏观麻风化病的主要机制。 然而,CD47依赖机制的定义不完善。 在此,我们报告了SLAM家庭接受者(SFRs)的关键作用,令人感到惊讶的是,他特有的剖腹泻电池和形成同类互动,以抑制宏观麻风化症。 我们发现,SFR的缺陷引发了肝炎的肝炎脂,导致严重拒绝了捐助者在受援的水里的乙型肝炎。 主要的SLAMF3和SLAMF4等特定的SFR成员被确定为“绿色餐饮”对宏观麻风的接受者。 这些接受者阻止了“我”的信号,例如LRP1-media宣布通过SH2区装灌皮霍斯法。 社区团结协会与但独立于CD47,以减轻宏观麻风化症,并联合删除了SFRs和CD47,导致在水里实行了乙酸化的实验室。 这种以恒心血管疾病的病人中,患有精神病患者的心理创伤。 综合症是一种不力的肝炎,其特点是肝炎对乙型肝炎电池的不力。 SFRs的贷款可能引起对乙型酸盐的否决。 采用反垄断制度也大大提高了CD19号抗体阳性肝炎目标的力度,其宏观成套显示裁谈会19个反根概念。 因此,对乙型肝炎菌菌的抑制对乙型肝炎至关重要,而SFRs可能代表以前的戒毒治疗目标。

SLAMF3 and SLAMF4 are immune checkpoints that constrain macrophage phagocytosis of hematopoietic tumors

SLAM family receptors (SFRs) are widely expressed on hematopoietic cells, and SFRs have been implicated in various innate immune functions. Here, Li et al. used various knockout cells and mice to show that SFR deficiency, specifically SLAMF3 and SLAMF4, increases the ability of macrophages to phagocytose hematopoietic cells. These SFRs reduced LRP1-mediated activation of mTOR and Syk signaling to inhibit said macrophages. SFR knockout mice exhibited hematopoietic tumor rejection, and SFR deletion was able to enhance the efficacy of CAR-macrophage therapy against hematopoietic cancer. Together, these data show that SFR expression on hematopoietic cells can inhibit the function of macrophages, pointing to a potential therapeutic target for hematopoietic cancers. The interaction of SIRPα with CD47 represents a major mechanism for preventing macrophage phagocytosis. However, CD47-independent mechanisms are poorly defined. Here, we report a critical role of SLAM family receptors (SFRs), ubiquitously
science.org/doi/abs/10.1126/sc


SARS-CoV-2 Omicron变:反人蒸气和蒸汽-EM结构

新报告的奥蒂龙变体将把“三角洲”改为全世界最普遍的SARS-CoV-2变量。 环里奥-EM对奥地变量的结构性分析与人类ACE2相复杂的蛋白质显示,在经常预算与ACE2进行混合残留物的R493、S496和R498形成的新的盐桥和氢债券。 这些互动似乎比其他地补偿,例如K417N已知减少ACE2具有约束力的硫化物,从而产生了类似的生物化学ACE2对Data和Omicron变量具有约束力的照相。 光衣服显示,在Omicron le 蛋白质上展示的假病毒增加了反光。 反人逃亡的增多,以及保留在ACE2接口的强有力互动,因此,是可能有助于快速蔓延奥地变量的重要的分子特征。

SARS-CoV-2 Omicron variant: Antibody evasion and cryo-EM structure of spike protein–ACE2 complex

The newly reported Omicron variant is poised to replace Delta as the most prevalent SARS-CoV-2 variant across the world. Cryo-EM structural analysis of the Omicron variant spike protein in complex with human ACE2 reveals new salt bridges and hydrogen bonds formed by mutated residues R493, S496 and R498 in the RBD with ACE2. These interactions appear to compensate for other Omicron mutations such as K417N known to reduce ACE2 binding affinity, resulting in similar biochemical ACE2 binding affinities for Delta and Omicron variants. Neutralization assays show that pseudoviruses displaying the Omicron spike protein exhibit increased antibody evasion. The increase in antibody evasion, together with retention of strong interactions at the ACE2 interface, thus represent important molecular features that likely contribute to the rapid spread of the Omicron variant.

Science, Ahead of Print.
science.org/doi/abs/10.1126/sc


传真:2 基于AlGaAs阶段的敏感amplifier,有记录收益、噪音和敏感性。

阶段敏感的纤维纤维纤维纤维纤维纤维纤维纤维器有可能在新一代的光学通信系统以及声音处理的品质和量上取得实质性进展。 虽然第二令非线性(TM2)比第三秩序更强,但由于有效阶段与互动浪相匹配的挑战,在半导体内很少使用。 在这项工作中,我们表明我们的知识成功设计、构造和定性,第一版是以半导体为基础的泛美测量系统,在阿格拉阿斯·卜拉格波茨采用有效的阶段匹配办法和压缩泵。 据报告,AlGaAs PSA在进入30个B阶段获得收益,每批505个摄影师敏感。 其业绩还处理了0.亿比的理论最低噪音数字。 由于这种业绩衡量及其在单一模式制度中运作的能力,本方案可以继承一个关于数量的渠道的新时代。

χ^2-based AlGaAs phase sensitive amplifier with record gain, noise, and sensitivity

Phase sensitive amplifiers (PSAs) have the potential to empower substantial advances in emerging generations of optical communication systems as well as classical and quantum on-chip signal processing. While second order nonlinearity (χ2) is stronger than third order nonlinearity (χ3), it is seldom utilized in semiconductors to realize PSAs owing to the challenges of effectively phase matching the interacting waves as well as countering the two-photon absorption of the pump. In this work, we demonstrate the successful design, fabrication, and characterization of, to our knowledge, the first χ2-based semiconductor PSA using an efficient phase matching approach and a pulsed pump, in an AlGaAs Bragg reflection waveguide. The reported AlGaAs PSA achieves on-chip in-phase gain approaching 30 dB, with a sensitivity of 0.005 photons per pulse. Its performance also approaches the theoretical minimal noise figure of 0 dB. With such performance metrics and its capability to operate in the single mode regime, this PSA could usher in a new era of on-chip quantum circuits.

Zhizhong Yan, Haoyu He, Han Liu, M. Iu, O. Ahmed, E. Chen, P. Blakey, Youichi Akasaka, T. Ikeuchi, Amr S. HelmyPhase sensitive amplifiers (PSAs) have the potential to empower substantial advances in emerging generations of optical communication ... [Optica 9, 56-60 (2022)]
osapublishing.org/abstract.cfm


近乎寻常的图像分析显示,全球濒危信号伙伴网络,包括国家定位系统-RhoGEF。

i 互动式 代理的胸部骨干器刺激了G蛋白质(α)的子弹药,以诱导在轻微的神经恋童癖者和纤维的电池中。 为查明活跃的Gi 、Chandan等人的效应者,在表示活跃和活跃的G类的电池中进行了接近的标签和大规模的谱测量分析。 在作者确定的候选人中,由于其以前曾确定过的因Gamili-coledrechttractrent 接受者而在神经中作用,RhoGEF PRG特别感兴趣。 在人类微不足道的野生生物中,在启动红色彩的抗药性政策审查1时,需要Givi对RhoA活动进行干预。 因此,可以使用作者的排他办法,确定与在手术室内的其他蛋白质子单位互动的有约束力的伙伴。 G 乙酸脂被带到G级G蛋白质的家庭是电池和组织物理学的主要监管者。 我们以前的工作表明,在管制流血者和菲罗萨迪加电池的移徙方面,Givi在活的支票上发挥了新的作用。 在此,我们使用了一个光电池,把它称为“红色彩色”(TMT)的定量定性分类分析,以查明有选择地与GTP-有约束力的甘米形式互动的蛋白质。 查明并验证了多种目标,与生物开发2-传染性相适应,形成活跃的Kavi1 mutant,建议在活性电池中建立一个活性接触网。 我们表明,尽管全球蛋白质量超过85%,但活跃的Givi1 ,但不是Givi2 ,刺激了一名候选人的蛋白质。 我们还表现在人类核心恋童癖者中,他们积极活跃。 大约几千米可能制约了磷酸化的Iosin轻型链,这是一个对移徙至关重要的进程,通过启动知识产权方案。 查明和定性由G类群直接或间接管制的新目标,将有助于调查Gamili-coupled GPCRs在多种生物进程中的功能作用。

A network of Gαi signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEF

i interactome Activated chemoattractant receptors stimulate the G protein α i subunit to induce de-adhesion in migrating neutrophils and fibrosarcoma cells. To identify effectors for active Gα i , Chandan et al. performed proximity labeling and mass spectrometry analysis in cells expressing inactive and constitutively active Gα i . Of the candidates identified by the authors, the RhoGEF PRG was of particular interest because of its previously identified role in neutrophil chemotaxis induced by the activation of Gα i -coupled chemoattractant receptors. In human neutrophils, Gα i was required for PRG-mediated stimulation of RhoA activity upon activation of the chemoattractant receptor FPR1. Thus, the authors’ approach could be used to identify binding partners that interact with other G protein subunits in intact cells. G protein–coupled receptors (GPCRs) that couple to the Gα i family of G proteins are key regulators of cell and tissue physiology. Our previous work has revealed new roles for Gα i in regulating the migration of neutrophils and fibrosarcoma
science.org/doi/abs/10.1126/sc


BNT162b2米的BNTS-CoV-2 Omicron的断肢

全球分布的SARS-CoV-2 Variant关切Omicron(B.1.1.529)比任何时候都多,特别是在蛋白质的蒸发方面,这表明通过消除反bo风的承认可能受到损害。 我们测试了51名与会者的Wuhan、Beta、 Delta或Omicron假病毒,他们接受了两间或三发的米歇尔-米歇尔-COVID-19疫苗BNT1622。 在两起疫苗之后,222倍的投机比Wuhan假病毒相比减少了对Omicron的投机。 第三个疫苗接种后一个月,与两个剂量相比,增加了23倍的肺炎剂,与两剂量相类似,在两剂量之后的丙醇投机者也增加了。 在参与者的子体中,使用SARS-CoV-2的第三个疫苗接种有效消除奥地的要求得到了证实。 这些数据表明,三发的米歇尔疫苗BNT162b2可以保护,以免被诱导的COVID-19。

Neutralization of SARS-CoV-2 Omicron by BNT162b2 mRNA vaccine–elicited human sera

The globally-circulating SARS-CoV-2 Variant of Concern Omicron (B.1.1.529) has a large number of mutations especially in the spike protein, indicating that recognition by neutralizing antibodies may be compromised. We tested Wuhan, Beta, Delta, or Omicron pseudoviruses with sera of 51 participants that received two or three doses of the mRNA-based COVID-19 vaccine BNT162b2. Following two doses, sera had >22-fold reduced neutralizing titers against Omicron compared to Wuhan pseudovirus. One month after the third vaccine dose, Omicron-neutralizing titers were increased 23-fold compared to two doses, with titers similar to Wuhan-neutralizing titers after two doses. The requirement of a third vaccine dose to effectively neutralize Omicron was confirmed using live SARS-CoV-2 in a subset of participants. These data suggest that three doses of the mRNA vaccine BNT162b2 may protect against Omicron-mediated COVID-19.

Science, Ahead of Print.
science.org/doi/abs/10.1126/sc


查明严重形式的COVID-19的驱动力

由于我们更深刻地了解了2019年(COVID-19)的常见性病毒疾病的驱动因素,必须从不同人口的角度来理解这些驱动因素,包括那些被认为面临严重疾病危险的人。 在此,卡普托等人。 采用多类方法,确定青年、无道德病人的急诊器中的重要COVID-19的司机。 作者利用各种机器学习、深入学习、定量和结构性因果模式,确定多位候选人发电机,包括金属板、金属环联会9混合。 这些调查结果共同表明,关键COVID-19的驱动因素,因此,治疗方式可能因合谋而异。 2019年(COVID-19)关键共同疾病的司机仍然不明。 鉴于诸如年龄和共同道德等主要因素,这一条件的真正调解人仍然很不确定。 我们利用多类分析,并在新病人的门诊器中使用人为情报,在幕后排除了主要的纪念碑。 共同点包括47个“关键”(在机械预防的密集护理单位)和25个“非道德”病人(非关键照料)和有COVID-19和22个健康的个人。 分析包括整个基因组、全方红的自然资源、异类和血单核电池、骨干特征描述和高能的天基杀。 使用了一所机学习、深入学习、定量和结构性因果模式。 严重COVID-19的病人的特点是:煽动性加剧、动荡的残害和我的教训、增加胁迫和维生机生物学。 在有区别表示的慷慨中,我们注意到对金属投机的管制。 这种慷慨签字在第二个独立的共体上得到证实,有81名重要和73名被带到COVID-19的病人,并在记录和蛋白质水平以及辅助活动中得到进一步证实。 维生素A9 阻挡严重呼吸道综合症(SARS-CoV-2) 人体肺病电池的吸入和复制。 最后,在有COVID-19的个人的青年人中,我们提供了生动植物群的景观,那里的独特的签名与非关键病人有区别。 我们进一步确定,医师协会9是疾病的严重性和候选人治疗对象。

Identification of driver genes for critical forms of COVID-19 in a deeply phenotyped young patient cohort

As we gain more insight into the drivers of coronavirus disease 2019 (COVID-19), it is essential that these drivers be understood in the context of different populations, including those thought to be at low risk of developing severe disease. Here, Carapito et al. used a multi-omics approach to identify drivers of critical COVID-19 in a young, comorbidity-free patient cohort. The authors used an ensemble of machine learning, deep learning, quantum annealing, and structural causal modeling to identify multiple candidate driver genes, including the metalloprotease, ADAM9 . Together, these findings suggest that drivers of critical COVID-19, and thus treatment, may differ based on the cohort. The drivers of critical coronavirus disease 2019 (COVID-19) remain unknown. Given major confounding factors such as age and comorbidities, true mediators of this condition have remained elusive. We used a multi-omics analysis combined with artificial intelligence in a young patient cohort where major comorbidities were excluded at the onset. The
science.org/doi/abs/10.1126/sc


合作分散的次导记忆

电磁场与随机结构事项之间的互动是许多自然现象的核心。 在稳定的国家,其微星描述涉及由于物质系统的存在而对即将到来的辐射和实地进行定位。 由于互惠,这一次排放表明是一个突出的连贯特点,导致后流强度的提高。 当事件现场突然关闭,因为结果取决于辐射的空间和时间性质,这种图像就很复杂。 在此,我们表明,在事件辐射停止在空间受约束的中长期存在现场的朝向记忆。 我们的结果表明,由于电磁场主要位于中型喷雾器附近,出现了一个连贯的后继。 这些模式是一般性的,是对与严重分散的事务进行富足的照明交流的补充。 这种长期生存的、对等候的辐射的诱惑和空间财产取决于各种不成见,而这种污染为记忆应用开辟了新的机会。

Subradiant directional memory in cooperative scattering

The interaction between electromagnetic fields and randomly structured matter is at the core of many natural phenomena. In the steady state, its microscopic description involves the superposition of the incoming radiation and the field due to the presence of the material system. Owing to reciprocity, this secondary emission manifests a striking coherent feature leading to an enhancement of backward intensity. This portrayal is complicated when the incident field is suddenly shut off since the outcome depends on both the spatial and temporal properties of radiation. Here we demonstrate that a directional memory of the incoming field persists long after the incident radiation ceases to be present within a spatially bounded medium. Our results reveal that a coherent back-emission emerges as a result of subradiant quasi-modes of an electromagnetic field located primarily in the vicinity of the medium’s interface. The paradigm is general and complements the rich phenomenology of light interaction with strongly scattered matter. The temporal and spatial properties of this long-lived, reciprocity-maintaining radiation depend on the type of illumination, which opens up new opportunities for memory applications.

Nature Photonics, Published online: 10 January 2022; doi:10.1038/s41566-021-00926-4A theoretical study of the time dependence of backscattering enhancements is presented, where strongly scattering media present a directional memory for time-varying signals even when the direct input signal has long decayed, providing opportunities for co
nature.com/articles/s41566-021


无一机机的构件和储存

植根可能有助于加速生物制人为组织从研究院向诊所建造。 这个方法帮助生物工程师构造细胞组织,并把它们存放在长期。 这一特点增加了生物制构体的可利用性。 试验样品是使用GelMA-基于水力的水格、CPA和各种人类电池的生物印样。 我们的调查结果表明,在液体氮的蒸气和湿度3个月之后,该电池仍然可行和功能。

Freeform cell-laden cryobioprinting for shelf-ready tissue fabrication and storage

Cryobioprinting potentially empowers the acceleration of the transition of biofabricated artificial tissue constructs from research labs to clinics. The method helps bioengineers to fabricate cell-laden tissue constructs and store them for extended periods of time. This feature adds more flexibility in shelf availability of biofabricated tissue constructs. The test samples were bioprinted using GelMA-based hydrogels, CPAs, and various human cells. Our findings showed that the cells remained viable and functional after 3 months of cryobioprinting and cryopreservation in liquid nitrogen.

Cryobioprinting potentially empowers the acceleration of the transition of biofabricated artificial tissue constructs from research labs to clinics. The method helps bioengineers to fabricate cell-laden tissue constructs and store them for extended periods of time. This feature adds more flexibility in shelf availability of biofabricated tissue constructs. The test samples were bioprinted using GelMA-based hydrogels, CPAs, and various human cells. Our findings showed that the cells remained viable and functional after 3 months of cryobioprinting and cryopreservation in liquid nitrogen.
cell.com/matter/fulltext/S2590


出版: 多样化的光谱

Publisher Correction: Ever-evolving optical lattice clocks

Nature Photonics, Published online: 10 January 2022; doi:10.1038/s41566-022-00954-8Publisher Correction: Ever-evolving optical lattice clocks
nature.com/articles/s41566-022


计算机辅助设计可逆转的混合化链反应(CAD-HCR)使多功能的单一空间测量系统化

在现场对单一单位进行空间分类分析尚未实现,主要原因是目前技术的投入和敏感性不足。 最近关于天免疫核心酸化技术的进步为解决这一问题提供了巨大机会。 在此,我们报告了一个创新的混合系统反应(HCR)技术,涉及计算机辅助设计和可逆转的集会。 社会福利会能够拥有一个能够平行工作的顺序数据库,而不可逆转的集会使工作国与一个休息国之间能够改变HCR。 因此,为单一的空间学分析成功采用了CAD-HCR。 中性传播疾病的流感信号与传统天流感相类似,与目标蛋白质的丰度有积极联系,这对蛋白质的视化是有益的。 这里制订的方法扩大了单一优良分析和基因研究的工具箱,以及水文系统的表现和应用。

Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging

In situ spatial proteomics analysis of a single cell has not been achieved yet, mainly because of insufficient throughput and sensitivity of current techniques. Recent progress on immuno-nucleic acid amplification technology presents tremendous opportunities to address this issue. Here, we report an innovative hybridization chain reaction (HCR) technique that involves computer-aided design (CAD) and reversible assembly. CAD enables highly multiplexed HCR with a sequence database that can work in parallel, while reversible assembly enables the switching of HCR between a working state and a resting state. Thus, CAD-HCR has been successfully adopted for single-cell spatial proteomics analysis. The fluorescence signal of CAD-HCR is comparable with conventional immunofluorescence, and it is positively correlated with the abundance of target proteins, which is beneficial for the visualization of proteins. The method developed here expands the toolbox of single-cell analysis and proteomics studies, as well as the performance and application of HCR.

Science Advances, Volume 8, Issue 2, January 2022.
science.org/doi/abs/10.1126/sc


利用推土机进行汽车再生的诱惑

Osteoarthr炎是一项共同的条件,但治疗方式有限。 这里,Lu和同事制作了一种生物降解的扫描器,使用PLLA[poly(L-lactic))纳弗朗西斯,当被武力强加时,便产生一种宽恕,从而改善了维生素的风俗。 媒体在媒体中存在陈旧缺陷,在民解力量的扫描中,这表现了在1月或2个月之后,汽车再生和附属骨干重新抬头,以产生联合装载的错误。 虽然需要进一步努力优化分散的微观结构和组成部分,但这项研究提供了证据表明,生物降解的电子化管工作可以使用联合载荷的方式处理乙型肝炎。 超过3 350万美国成年人患有乙型肝炎,目前治疗,包括疼痛药品和抗药性药物,只会减轻症状,但并不治疗这一疾病。 在此,我们表明,在适用部队或联合装载的纳弗里克(L-lactic酸)纳弗朗布的生物降解器可以作为无电池的电器,促进风俗和汽车再生。 PLLA在适用部队或联合装载下进行扫描,产生一种可控制的光衣服,它推动了手机的蛋白质注射,便利了细胞移徙或招募,通过信号走路,诱导了内在的TGF-β,并改善了在维生和维生动中的传统和汽车循环。 在1至2个月的手术(包括1个月的手术前)之后,有高强度的脑膜缺陷,并进行有血管的整治,在1至2个月的手术(包括1个月的康复)之后,有全套的乙型肝炎和二丙型肝炎,而受非皮肤素的扫描和治疗的行李却遇到缺陷,并有限。 因此,将生物可降解的热液组织与受控制的机械化(六种实际操作)结合起来的做法,对于治疗乙型肝炎可能有用,并有可能适用于其他受害组织。

Exercise-induced piezoelectric stimulation for cartilage regeneration in rabbits

Osteoarthritis is a common condition, but curative therapies are limited. Here, Liu and colleagues developed a biodegradable scaffold using PLLA [poly( l -lactic acid)] nanofibers that when placed under applied force generated a piezoelectric charge, leading to improved chondrogenesis in vitro. Rabbits with osteochondral defects in the medial femoral condyle that were treated with the PLLA scaffold demonstrated improved cartilage regeneration and subchondral bone regeneration after 1 or 2 months of exercise to generate piezoelectric charge from the joint loads. Although further work is needed to optimize the scaffold microstructure and components, this study provides evidence that biodegradable piezoelectric scaffolds can use joint-loading exercise to treat osteoarthritis. More than 32.5 million American adults suffer from osteoarthritis, and current treatments including pain medicines and anti-inflammatory drugs only alleviate symptoms but do not cure the disease. Here, we have demonstrated that a biodegradable piezoelectric poly( l -lactic acid) (PLLA) nanofiber scaffold under applied force or joint load could act as a battery-less electrical stimulator to promote ch
science.org/doi/abs/10.1126/sc


纵向分析显示,由于多种微不足道的疾病,注射病毒的流行率很高。

多方面的疏散症是中央神经系统长期的煽动性疾病。 我们试验了Spstein-Barr病毒(EBV)在一手旁造成该地的假设,其中955人在服兵役期间被诊断到该地。 在感染艾滋病后,MS的风险增加了32倍,但与其他病毒相比,包括类似的传染性活性病毒,也不会增加。 神经流感链的谣层,即神经生物标志,只在乙酸化后增加。 这些调查结果不能由任何已知的MS风险因素加以解释,并建议欧洲太平洋共同体作为MS的主要原因。

Longitudinal analysis reveals high prevalence of Epstein-Barr virus associated with multiple sclerosis

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system of unknown etiology. We tested the hypothesis that MS is caused by Epstein-Barr virus (EBV) in a cohort comprising more than 10 million young adults on active duty in the US military, 955 of whom were diagnosed with MS during their period of service. Risk of MS increased 32-fold after infection with EBV but was not increased after infection with other viruses, including the similarly transmitted cytomegalovirus. Serum levels of neurofilament light chain, a biomarker of neuroaxonal degeneration, increased only after EBV seroconversion. These findings cannot be explained by any known risk factor for MS and suggest EBV as the leading cause of MS.

Science, Ahead of Print.
science.org/doi/abs/10.1126/sc


传真:2 基于AlGaAs阶段的敏感amplifier,有记录收益、噪音和敏感性。

阶段敏感的纤维纤维纤维纤维纤维纤维纤维纤维器有可能在新一代的光学通信系统以及声音处理的品质和量上取得实质性进展。 虽然第二令非线性(TM2)比第三秩序更强,但由于有效阶段与互动浪相匹配的挑战,在半导体内很少使用。 在这项工作中,我们表明我们的知识成功设计、构造和定性,第一版是以半导体为基础的泛美测量系统,在阿格拉阿斯·卜拉格波茨采用有效的阶段匹配办法和压缩泵。 据报告,AlGaAs PSA在进入30个B阶段获得收益,每批505个摄影师敏感。 其业绩还处理了0.亿比的理论最低噪音数字。 由于这种业绩衡量及其在单一模式制度中运作的能力,本方案可以继承一个关于数量的渠道的新时代。

χ^2-based AlGaAs phase sensitive amplifier with record gain, noise, and sensitivity

Phase sensitive amplifiers (PSAs) have the potential to empower substantial advances in emerging generations of optical communication systems as well as classical and quantum on-chip signal processing. While second order nonlinearity (χ2) is stronger than third order nonlinearity (χ3), it is seldom utilized in semiconductors to realize PSAs owing to the challenges of effectively phase matching the interacting waves as well as countering the two-photon absorption of the pump. In this work, we demonstrate the successful design, fabrication, and characterization of, to our knowledge, the first χ2-based semiconductor PSA using an efficient phase matching approach and a pulsed pump, in an AlGaAs Bragg reflection waveguide. The reported AlGaAs PSA achieves on-chip in-phase gain approaching 30 dB, with a sensitivity of 0.005 photons per pulse. Its performance also approaches the theoretical minimal noise figure of 0 dB. With such performance metrics and its capability to operate in the single mode regime, this PSA could usher in a new era of on-chip quantum circuits.

Zhizhong Yan, Haoyu He, Han Liu, M. Iu, O. Ahmed, E. Chen, P. Blakey, Youichi Akasaka, T. Ikeuchi, Amr S. HelmyPhase sensitive amplifiers (PSAs) have the potential to empower substantial advances in emerging generations of optical communication ... [Optica 9, 56-60 (2022)]
osapublishing.org/abstract.cfm


A. 大量动态范围和广泛排出的与巴格纳的反转化探测

在室温室内直接发现中层喷雾器,通常会受到高度的背景噪音和缺少理想传感器材料的影响。 基于非线性变通桥梁的间接转用方法,使预期的马苏尔地区走到发达的设施。 在本文件中,我们报告对3 ± 微克米的解剖试验进行了试验研究,有1 微克米激光倾销了BaGa4Se7。 这一探查计划与光学器和光学接收器合并,以形成一个连贯的源和探测系统,提供高温度(第二年时间)和光谱解决(3cm-1)。 这种探险计划从这一非氧化物的上级特性中获益,并观察到1 0 fJ/pulse的最低限度可探测能源。 转换技术在敏感度(2 450 份)和波浪反应统一两方面外包了商业性化验器。 上述综合的IR源和探测系统的特点,使得对高分辨率的气体分析和光谱系统应用具有重大兴趣。

Large dynamic range and wideband mid-infrared upconversion detection with BaGa_4Se_7 crystal

Direct detection of mid-infrared (MIR) at room temperature commonly suffers from the high background noise and the lack of ideal sensor material. An indirect upconversion method based on nonlinear optics bridges the desired MIR region to the well-developed near-infrared devices. In this paper, we report an experimental study of 3–8 µm MIR upconversion detection with a 1 µm laser-pumped BaGa4Se7 crystal. This detection scheme is integrated with an optical parametric oscillator and an optical receiver to form a coherent source and detection system that offers high temporal (nanosecond time scale) and spectral resolution (≤3cm−1). The wide response band (>1.4 octave) and large dynamic range (11.5 orders) of this detection scheme benefits from the superior optical properties of this non-oxide crystal, and a minimum detectable energy of 1.02 fJ/pulse is observed with an InGaAs p-i-n photodiode. The upconversion technique outperforms commercial HgCdTe detectors in terms of both sensitivity (2–4.5 orders better) and wavelength response flatness. The aforementioned characteristics of this integrated MIR source and detection system make it of significant interest for applications in high-resolution gas analysis and spectral imaging.

Pengxiang Liu, Liyuan Guo, Feng Qi, Weifan Li, Wei Li, Qiaoqiao Fu, Jiyong Yao, Mingjun Xia, Zhaoyang Liu, Yelong WangDirect detection of mid-infrared (MIR) at room temperature commonly suffers from the high background noise and
osapublishing.org/abstract.cfm


Iospines en leetism and conigu极化的超级分化

在常规超级采样器中,在相反的电缆之间出现通力。 我们观察到伯尔·巴莱尔图尔的半极分化的超常性活动,然后再到一个悲的摇篮子。 大型产生的最独一无二的电力领域。 我们观察了电文门与电流层之间的电子阶段之间的过渡,这些阶段因其极分化而异,其空间是自由的附带和动力-空间温度的结合所界定的。 尽管所有这些阶段都是零磁场的金属,但我们认为,在B finite B NY 150T中,向一个超级采掘国过渡,与两个维权表相平行。 在不对称的突然过渡附近发生意外活动,完全存在于B.P.Pe de l, 预期将使用磁带的超级磁磁带,与观察到的温度T NY 30K号过渡,这符合附带的顺序。

Isospin magnetism and spin-polarized superconductivity in Bernal bilayer graphene

In conventional superconductors, Cooper pairing occurs between electrons of opposite spin. We observe spin-polarized superconductivity in Bernal bilayer graphene when doped to a saddle-point van Hove singularity generated by large applied perpendicular electric field. We observe a cascade of electrostatic gate-tuned transitions between electronic phases distinguished by their polarization within the isospin space defined by the combination of the spin and momentum-space valley degrees of freedom. Although all of these phases are metallic at zero magnetic field, we observe a transition to a superconducting state at finite B ‖ ≈ 150mT applied parallel to the two-dimensional sheet. Superconductivity occurs near a symmetry breaking transition, and exists exclusively above the B ‖ -limit expected of a paramagnetic superconductor with the observed transition temperature T C ≈ 30mK, consistent with a spin-triplet order parameter.

Science, Ahead of Print.
science.org/doi/abs/10.1126/sc


持续不断增长的多层水井结构,有无缝的中间接口

这里,报告采用一种方法,即超声径流式地推移地表催化剂性地开始激进的多金属化(UV-SCIRP),以准备各种温床的多环热器,与自然生物沉积物的增长路线相似,在地从一个水格层的表面上产生压力2+,以在会议室温下形成一个坚固的水利热层,以刺激激进的多金属化。 多种方法在建造复杂的水利格模式和任意形成的水格结构方面是有效的。

Continuously growing multi-layered hydrogel structures with seamless interlocked interface

Herein, a method, namely, ultraviolet-triggered surface catalytically initiated radical polymerization (UV-SCIRP), is reported for preparing layered structural hydrogels with a living polymerization growing process resembling the growth route of natural bio-tissues, in which Fe2+ ions were generated in situ from the surface-bound Fe3+ ions of a hydrogel substrate to catalyze radical polymerization at the solid-liquid interface to grow hydrogel layers at room temperature. The versatile method is effective in constructing complex hydrogel patterns and arbitrarily shaped layered hydrogel architectures.

Herein, a method, namely, ultraviolet-triggered surface catalytically initiated radical polymerization (UV-SCIRP), is reported for preparing layered structural hydrogels with a living polymerization growing process resembling the growth route of natural bio-tissues, in which Fe2+ ions were generated in situ from the surface-bound Fe3+ ions of a hydrogel substrate to catalyze radical polymerization at the solid-liquid interface to grow hydrogel layers at room temperature. The versatile method is effective in constructing complex hydrogel patterns and arbitrarily shaped layered hydrogel architectures.
cell.com/matter/fulltext/S2590

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